Case Study:

Transfection & Vaccines.



What was the challenge?

Internalisation of compounds, proteins or peptides in cells is an important method in immunologic studies. This may be relevant to studies on vaccine or drug development that require transfer of product into a target cell for expression of the product and/or activation of the cell for an immune response. The delivery of products may be carried out by carriers such as nanoparticles or viral vectors, or via active internalisation of the cell, or through cell transfection reagents.

Description R8 % R8 MFI

Dotted line

Dendritic cells (only) 0.0 83
Orange filled

Dendritic cells

+ load (non-fluorescent)

2.3 89

Grey filled

Dendritic cells

+ load (negative control)

2.5 69

Green filled

Dendritic cells

+ load (fluorescent)

69 1120


Studying vaccine or drug development that require transfer of product into a target cell for expression of the product and/or activation of the cell for an immune response.


We adopted a cell line based dendritic cell (DC) model to transfer mRNA carried by a nanoparticle carrier. Both fluorescent and non-fluorescent protein products (load) were transferred as mRNA to assess translation and availability of proteins in DCs by flow cytometry. 


There was a high availability of the fluorescent protein in the DCs.

Additional value:

This model can be used in various efficacy testing such as

  • Intracellular inhibitors that impact cell signalling and gene expression
  • New vaccines (protein or mRNA) in dendritic cell adaptive immunity
  • Drug availability in cells

Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.


Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

M1/M2 Polarisation

M1/M2 are important in studies of various therapeutic areas and biological pathways.



Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

Cytokine Release Assay

Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.


Dendritic Cell Maturation

Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 


T Cell Differentiation

The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.


Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.


Case Studies

Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.


Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.