Immune & Protein-Based Assays
We perform various immunologic and protein based assays that are needed in your research or drug development. While we provide standard assays our speciality is in developing custom designed assays suited for your product.
METHODS
- Co-cultures, co-culture in a chip/chamber
- Microscopy
- Flow cytometry
- ELISA
- Fluorescence and absorbance reading
- Western blotting
- Immunohistochemistry
- Mass spectrometry (small and large molecules)
- RNA interference (siRNA)
- Protein expression/ Transfection
- Surface plasmon resonance (SPR)
- Subcellular fractionation
- RNA extraction and quantitation
We use the methods for:
- Cell characterization (surface and intracellular marker expression)
- Cell culture fluid analysis for proteins, metabolites, cytokines and other biomarkers
- Cell lysate analysis
- In vitro immune assays
- Cell death analysis
- Tissue damage assessment
- Protein functional study
- Antibody applications testing
ASSAYS
We offer both PBMC-derived and human cell line-based assays.
- Dendritic cell (maturation) assay
- Th differentiation assay, such as Th1, Th2, Th17 assay
- T-cell activation assay
- Phagocytosis/ADCP assay/M1-M2 assay
- Cytokine secretion assay
- Mixed lymphocyte reaction assay
- Cytokine release assay
- NK cell assay
- NETosis
- ADCC
- Marker expression study
- Antigen specific T cell activation assay
- Cell death assay/ induced tissue damage
- Functional assay (gene knockdown and knock-in)
- Adhesion assay
- MTT assay
- LDH assay
- Cleaved caspase assay
- Scratch assay (wound healing)
- Immune and inflammation assays
- Human cell line assays
Please contact for more information.
APPLICATIONS (Immunology)
- Th differentiation: Th1, Th2, Th17
- Dendritic cell maturation
- T-cell activation
- DC-T cell interaction
- Phagocytosis
- Macrophage polarization
- Cytokine secretion profile
- Opsonisation and antibody dependent tumour phagocytosis
- Immune checkpoint study
- Cytokine release
- Mixed lymphocyte reaction
APPLICATIONS (Immunology)
- Th differentiation: Th1, Th2, Th17
- Dendritic cell maturation
- T-cell activation
- DC-T cell interaction
- Phagocytosis
- Macrophage polarization
- Cytokine secretion profile
- Opsonisation and antibody dependent tumour phagocytosis
- Immune checkpoint study
- Cytokine release
- Mixed lymphocyte reaction
APPLICATIONS (Cell & Protein)
- Drug treatment impact on protein expression
- Cell to cell interaction
- Migration of cells
- Cell attachment and spreading
- Cell proliferation
- Cell death and cytotoxicity
- Cell surface marker expression analysis
- Protein function (transfection or RNA)
- Protein expression and localisation
- Wound healing
Looking For A Tailor-Made Solution?
Leave us a message and we will be in touch shortly. We look forward to meeting you!