Immune & Protein-Based Assays

We perform various immunologic and protein based assays that are needed in your research or drug development. While we provide standard assays our speciality is in developing custom designed assays suited for your product.

 

METHODS

  • Co-cultures, co-culture in a chip/chamber
  • Microscopy
  • Flow cytometry
  • ELISA
  • Fluorescence and absorbance reading
  • Western blotting
  • Immunohistochemistry
  • Mass spectrometry (small and large molecules)
  • RNA interference (siRNA)
  • Protein expression/ Transfection
  • Surface plasmon resonance (SPR)
  • Subcellular fractionation
  • RNA extraction and quantitation

We use the methods for:

 

  • Cell characterization (surface and intracellular marker expression)
  • Cell culture fluid analysis for proteins, metabolites, cytokines and other biomarkers
  • Cell lysate analysis
  • In vitro immune assays
  • Cell death analysis
  • Tissue damage assessment
  • Protein functional study
  • Antibody applications testing

    ASSAYS

    We offer both PBMC-derived and human cell line-based assays. 

    • Dendritic cell (maturation) assay
    • Th differentiation assay, such as Th1, Th2, Th17 assay
    • T-cell activation assay
    • Phagocytosis/ADCP assay/M1-M2 assay
    • Cytokine secretion assay
    • Mixed lymphocyte reaction assay
    • Cytokine release assay
    • ADCC
    • Marker expression study
    • Antigen specific T cell activation assay
    • Cell death assay/ induced tissue damage
    • Functional assay (gene knockdown and knock-in)
    • Adhesion assay
    • MTT assay
    • LDH assay
    • Cleaved caspase assay
    • Scratch assay (wound healing)
    • Immune and inflammation assays
    • Human cell line assays

    Please contact for more information.

      APPLICATIONS (Immunology)

      • Th differentiation: Th1, Th2, Th17
      • Dendritic cell maturation
      • T-cell activation
      • DC-T cell interaction
      • Phagocytosis
      • Macrophage polarization
      • Cytokine secretion profile
      • Opsonisation and antibody dependent tumour phagocytosis
      • Immune checkpoint study
      • Cytokine release
      • Mixed lymphocyte reaction

          APPLICATIONS (Immunology)

          • Th differentiation: Th1, Th2, Th17
          • Dendritic cell maturation
          • T-cell activation
          • DC-T cell interaction
          • Phagocytosis
          • Macrophage polarization
          • Cytokine secretion profile
          • Opsonisation and antibody dependent tumour phagocytosis
          • Immune checkpoint study
          • Cytokine release
          • Mixed lymphocyte reaction

              APPLICATIONS (Cell & Protein)

              • Drug treatment impact on protein expression
              • Cell to cell interaction
              • Migration of cells
              • Cell attachment and spreading
              • Cell proliferation
              • Cell death and cytotoxicity
              • Cell surface marker expression analysis
              • Protein function (transfection or RNA)
              • Protein expression and localisation
              • Wound healing

                Looking For A Tailor-Made Solution?

                Leave us a message and we will be in touch shortly. We look forward to meeting you!

                Immune Modelling & Experimental Design

                Immune Risk Assessment

                Tissue Damage & Cytotoxicity

                Immuno-Oncology & Immunotoxicity

                Inflammation & Systemic Diseases

                Vaccines, Drug Delivery & Transfection

                Signalling Pathways

                Looking for a tailored solution?

                Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.

                 

                Some of our popular assays

                To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

                M1/M2 Polarisation

                M1/M2 are important in studies of various therapeutic areas and biological pathways.

                 

                ADCP

                Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

                Cytokine Release Assay

                Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.

                 

                Dendritic Cell Maturation

                Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 

                 

                T Cell Differentiation

                The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.

                 

                Immune & Protein-Based

                We perform various immunologic and protein-based assays that are needed in your research or drug development.

                 

                Case Studies

                Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.

                Blogs

                Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.