Dendritic Cell Maturation assay
Dendritic cells (DCs) are the star antigen presenting cells (APCs) of myeloid or haematopoietic origin that play a key role between the innate and adaptive immune systems. DCs are pivotal in the T cell mediated T-helper response to MHC ClassII-Ag complex, and also cross presenting via the MHC ClassI pathway. They consist of receptors for pathogen and danger associated molecular patternse.g. TRL’s.
In vitro Dendritic Cell (DC) Maturation Studies
We generate DCs from a human monocytic cell line or derive DCs from primary monocytes or haematopoietic cells. These cells express CD11c, HLA-DR, CD80, CD86, SIRPa, CD14 and can be used in mono or co-cultures to study drug effects for DC maturation and various DC related outcomes. DCs can be characterised by flow cytometry, immunohistochemistry and microscopy and also through cytokine measurements. We can perform assays with a T cell line for immune functional studies.
Applications
DCs are very important in studies of various therapeutic areas and biological pathways:
- Tumour biology and oncology
- Wound healing
- Infection
- Inflammatory and autoimmune conditions
- Cardiovascular disease
- Tissue damage and cell death
In recent years much attention has been given to in vitro DC based models in cancer studies.
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