Th17 Differentiation and Assay

Upon antigen processing and maturation the dendritic cell-MHC Class II complex interacts with CD4 T cells with a TCR activation response that leads to the differentiation of T-helper (Th) cells. One of the pro-inflammatory differentiation of Th cells is the Th17 that is associated with the production of IL-17, IL-22, IL-21 and IL-26. Cytokines that contribute to Th17 development are TGFbeta, IL-6, IL-21 and IL-23. The transcription factors STAT3, RORgamma  and RORalpha are key factors in the Th17 differentiation.

 

In vitro Th17  Differentiation Assay

Our in-house human T cell assay is adapted to differentiate Th17 cells from TALL-1 cells. On stimulation (e.g. with PMA) these cells produce IL-17 and IL-22 which are quantified by intracellular flow cytometry or ELISA. This assay can be performed in 96-well plates to test the effect of compounds on Th17 differentiation and activation (e.g. inhibitory effect) and on the viability of Th17 cells. The assay can be also be performed with primary T cells.

Applications

The Th17 pathway is important in studies of various therapeutic areas and biological pathways:

  • Infection
  • Autoimmunity
  • Tumour biology and oncology
  • Cardiovascular disease
  • Tissue damage and cell death

In recent years the need to develop and source human cell based Th17 assay has increased.

Looking For A Tailor-Made Solution?

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Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.

 

Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

M1/M2 Polarisation

M1/M2 are important in studies of various therapeutic areas and biological pathways.

 

ADCP

Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

Cytokine Release Assay

Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.

 

Dendritic Cell Maturation

Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 

 

T Cell Differentiation

The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.

 

Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.