Upon antigen processing and maturation the dendritic cell-MHC Class II complex interacts with CD4 T cells with a TCR activation response that leads to the differentiation of T-helper (Th) cells. One of the pro-inflammatory differentiation of Th cells is the Th17 that is associated with the production of IL-17, IL-22, IL-21 and IL-26. Cytokines that contribute to Th17 development are TGFbeta, IL-6, IL-21 and IL-23. The transcription factors STAT3, RORgamma and RORalpha are key factors in the Th17 differentiation.
In vitro Th17 Differentiation Assay
Our in-house human T cell assay is adapted to differentiate Th17 cells from TALL-1 cells. On stimulation (e.g. with PMA) these cells produce IL-17 and IL-22 which are quantified by intracellular flow cytometry or ELISA. This assay can be performed in 96-well plates to test the effect of compounds on Th17 differentiation and activation (e.g. inhibitory effect) and on the viability of Th17 cells. The assay can be also be performed with primary T cells.
The Th17 pathway is important in studies of various therapeutic areas and biological pathways:
- Tumour biology and oncology
- Cardiovascular disease
- Tissue damage and cell death
In recent years the need to develop and source human cell based Th17 assay has increased.
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