Th17 Differentiation and Assay

Upon antigen processing and maturation the dendritic cell-MHC Class II complex interacts with CD4 T cells with a TCR activation response that leads to the differentiation of T-helper (Th) cells. One of the pro-inflammatory differentiation of Th cells is the Th17 that is associated with the production of IL-17, IL-22, IL-21 and IL-26. Cytokines that contribute to Th17 development are TGFbeta, IL-6, IL-21 and IL-23. The transcription factors STAT3, RORgamma  and RORalpha are key factors in the Th17 differentiation.


In vitro Th17  Differentiation Assay

Our in-house human T cell assay is adapted to differentiate Th17 cells, either isolated from PBMCs or generated from TALL-1 cells. On stimulation (e.g. with PMA) these cells produce IL-17 and IL-22 which are quantified by intracellular flow cytometry or ELISA. This assay can be performed in 96-well plates to test the effect of compounds on Th17 differentiation and activation (e.g. inhibitory effect) and on the viability of Th17 cells. The assay can be also be performed with primary T cells.


The Th17 pathway is important in studies of various therapeutic areas and biological pathways:

  • Infection
  • Autoimmunity
  • Tumour biology and oncology
  • Cardiovascular disease
  • Tissue damage and cell death

In recent years the need to develop and source human cell based Th17 assay has increased.

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Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.


Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

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Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.


Case Studies

Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.


Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.