Antibody Dependent Cellular Phagocytosis (ADCP) Assays

Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions that is mediated by leukocytes such as macrophages, DCs, neutrophils, etc. One of the types of phagocytosis is antibody-dependent cellular phagocytosis (ADCP) in which antibody-opsonised target cells activate phagocytes via Fc-FcR binding. This leads to internalization and degradation of the target cell which is a useful mechanism in infection and tumour biology, and the basis of many antibody-based drugs.


In vitro ADCP phagocytosis Assays

Our in-house phagocytosis assay using PBMC-derived or cell line-based macrophages is adapted to an ADCP assay where the target cell is co-cultured with phagocytes. In addition to generating microscopy based co-localisation readings of target cell and phagocyte (representing cell attachment) we generate flow cytometry based phagocytosis readings in which the values of the phagocytosed vs non-phagocytosed populations can be quantified. In this model the target cell can be an adherent or a suspension cell.

Applications of ADCP Assays

ADCP is important in studies of various therapeutic areas and biological pathways:

  • Tumour biology and oncology
  • Infection
  • Immuno oncology
  • Tissue damage and cell death

In recent years much attention has been given to in vitro ADCP models in oncology.

Looking For A Tailor-Made Solution?

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Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.


Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

M1/M2 Polarisation

M1/M2 are important in studies of various therapeutic areas and biological pathways.



Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

Cytokine Release Assay

Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.


Dendritic Cell Maturation

Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 


T Cell Differentiation

The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.


Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.


Case Studies

Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.


Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.