Case Study:

Inflammatory & Systemic Conditions



What was the challenge?

The immune system is central to responding to and mediating inflammatory, systemic or autoimmune conditions. Inflammatory conditions may rise from pathogenic infections, injuries or metabolites from internal dysregulations. Systemic conditions may rise from genetic causes, dysregulated systems or certain intolerances in metabolic pathways. Therapeutics are targeted to neutralise or inhibit acute or chronic immune responses that are associated with innate immune (such as phagocytosis, macrophage polarisation) or specific T-helper pathways (such as Th1, Th2 or Th17).

differentiated activated inhibited R5 % R5 MFI R7 % R7 MFI
Black line X X 1.4 59.8 13.8 25.4
Yellow filled X 15.5 57.6 80.3 33.8
Green filled 5.8 61.4 64.9 27.2


To test the ability of a compound to inhibit Th17 response in an inflammatory condition.

Approach: in vitro Th17 differentiation assay

We adopted our in-house human Th17 assay to test multiple formulations in a dose-dependent manner for inhibition of a Th17 response. TALL-1 cells differentiated and activated were treated with the formulations and measured for IL-17 and IL-22 by flow cytometry.


Effective formulations were identified and research proceeded to clinical studies.


Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.


Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

M1/M2 Polarisation

M1/M2 are important in studies of various therapeutic areas and biological pathways.



Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

Cytokine Release Assay

Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.


Dendritic Cell Maturation

Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 


T Cell Differentiation

The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.


Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.


Case Studies

Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.


Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.