Case Study:

Immune Modelling & Experimental Design.



What was the challenge?

Studies in experimental immunology commonly involve complex immune pathways that cannot be tested by single or standard assays. The designing of the experiment in a model is necessary with the consideration and knowledge of multiple factors in the signalling cascade, the interaction of multiple cells and their immunogenic or tolerogenic response.


Assess if danger signals can affect the initiation of an autoimmune response.


HSC70, a chaperone was chosen as a constitutively expressed protein that acts as a danger signal. An engineered in vivo model was designed with the over expression of HSC70 in beta islet cells only, and Ag-specific CD8+ T cells. In a sterile inflammation, diabetes was induced with targetted islet beta cell damage. Incidence of diabetes was measured, and DC and T cell responses were determined to assess immune tolerance versus immune activation leading to autoimmunity.


  • Initial beta cell death was responsible for the initiation of an immune mediated diabetes
  • The immune response was increased by the over-expression of danger signal

Added Value:

  • This model can be adapted into human cell based modules for translational data,
  • Intervention points for treatments can be tested,
  • Mechanistic data on signaling pathways for drug targeting can be generated.


Immune Modelling & Experimental Design

Immune Risk Assessment

Tissue Damage & Cytotoxicity

Immuno-Oncology & Immunotoxicity

Inflammation & Systemic Diseases

Vaccines, Drug Delivery & Transfection

Signalling Pathways

Looking for a tailored solution?

Of course, there is no one-size-fits-all solution for most studies. Immundnz specializes in developing tailored assays to enable you to challenge the status quo in your research.


Some of our popular assays

To accelerate your research and deliver high-quality data, Immundnz offers a broad spectrum of standardized, proven assays that are readily available.

M1/M2 Polarisation

M1/M2 are important in studies of various therapeutic areas and biological pathways.



Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils. 

Cytokine Release Assay

Cytokine release assay (CRA) is vital in biopharmaceutical discovery and development and life science research.


Dendritic Cell Maturation

Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems. 


T Cell Differentiation

The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds.


Immune & Protein-Based

We perform various immunologic and protein-based assays that are needed in your research or drug development.


Case Studies

Immundnz has performed many case studies, some of which are described here. You can always reach out to us for other examples of projects we have done.


Immundnz shares insights on in vitro immunology research for drug discovery on its blog, which is regularly updated by experts in the field.